POOR QUALITY EMBRYOS IMPLANTATION POTENTIAL
Cristina Torrado, Marta Guijarro, María M. Hebles, Mónica Dorado, Javier Ávila-Medina, Pascual Sánchez.
aClínicas Ginemed Sevilla. Farmacéutico Murillo Herrera, 3. 41010, Seville. Spain; bClínicas Ginemed Huelva. Calle Punta Umbría, 8. 21002 Huelva, Spain.
The aim of our study was to determine whether extended culture of surplus embryos that are not cryopreserved on day 3 because of not enough quality according to ASEBIR embryo grading, are capable to reach blastocyst stage, with optimum quality to be cryopreserved and transferred if needed during the following cycle.
1462 stimulated cycles performed in “Clínicas Ginemed” in 2016 from 1372 patients were analysed in a retrospective study. Best quality embryo/s were transferred on day 3 and supernumerary good quality ones were vitrified. Blastocysts originated from poor quality embryos (graded as D on day 3) were vitrified only if trophectoderm and inner cell mass were graded between A and C.
5.1% of all poor quality embryos obtained from 1462 stimulated cycles reached blastocyst, of those 46.25% were graded as B and 53.75% as C, and vitrified ;being 11 of them transferred after 100% warming survival, reaching an ongoing pregnancy on 45.4% cases (5 out of 11) of which 2 miscarriages between the seventh and eight week. And the final new born rate were 27.3%.
Surplus embryos that would be discarded following morphological criteria could have a second chance of being transferred if they were grown until day 5 or 6 of embryo culture, developing and implanting with a frequency reasonable of baby born with embryos who could be discarded in early developing stage.